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APPENDIX III

BENTHIC INVERTEBRATE ABUNDANCE DATA
AND
SEDIMENT TOXICITY TEST RESULTS

Appendix III.1 Benthic Invertebrate Abundance Data

Benthic Invertebrate Abundance Data for River Sites, Moira River System, Spring 1999

Waterbody	Black R.			Skootamatta R.			Salmon R.			Salmon R.			Salmon R.			Young's Ck.			Moira R.
TAXON      Site	BR-1	BR-1	BR-1	SKR-1	SKR-1	SKR-1	SR-1	SR-1	SR-1	SR-2	SR-2	SR-2	SR-3	SR-3	SR-3	YC-1	YC-1	YC-1	MR-1	MR-1	MR-1
Replicate	A	B	C	A	B	C	A	B	C	A	B	C	A	B	C	A	B	C	A	B	C
COELENTERATA:
HYDRIDAE:
Hydra
PLATYHELMINTHES:
PLANARIIDAE:
"microturbellaria"
NEMERTEA:
Prostoma	8		4	8
NEMATODA:	16		12	16	64	32	32	8	96	16	20		8	4	4				32	56	60
ANNELIDA:
HIRUDINEA:
ERPOBDELLIDAE:
Mooreobdella fervida
GLOSSIPHONIIDAE:
Deserobdella phalera
Helobdella elongata				8
OLIGOCHAETA:
ENCHYTRAEIDAE:				48	64	8	136	24	80	16		4	24	8	4
LUMBRICULIDAE:
Lumbriculus variegatus							8		8			4
NAIDIDAE:
Arcteonais lomondi		16
Dero digitata	24	32	12				24	32		8			8	4			16
Haemonais waldvogeli
Nais communis
Nais variabilis
Ophidonais serpentina								24	8
Pristina aequiseta									8								8
Slavinia appendiculata
Stylaria lacustris
SPARGANOPHILIDAE:
Sparganophilus eiseni				24	8
TUBIFICIDAE:
Aulodrilus americanus
Aulodrilus limnobius
Aulodrilus pluriseta
Ilyodrilus templetoni					24		16	24	40	8	4	8							176		284
Limnodrilus spp.	16	24	24	32	16	56	392	240	264		32		32		4				52	124	72
Limnodrilus claparedianus									8	8										24	12
Limnodrilus hoffmeisteri					8	24	32	24	24		4		8						176	24	112
MOLLUSCA:
BIVALVIA:
SPHAERIIDAE:
Prisidium	16	48	4	88	128	72	8			32	24	16		4	8				16	32	112
Sphaerium striatinum	4										16
GASTROPODA:
ANCYLIDAE:
Ferrissia													4
HYDROBIIDAE:
Amnicola limosa	32	8	4			8	8				8									4
Amnicola walkeri
Probythinella lacustris
LYMNAEIDAE: (juvenile)
Pseudosuccinea columella
PHYSIDAE:
Physella gyrina					8
PLANORBIDAE:
Gyraulus deflectus
Gyraulus parvus			4	8						4	4			4
Helisoma anceps
VALVATIDAE:
Valvata tricarinata		8				8
VIVPARIDAE:
Campeloma decisum																					4
Viviparus georgianus
ACARINA:	8					8				4
CRUSTACEA:
AMPHIPODA:
CRANGONYCTIDAE:
Crangonyx					8
HYALELLIDAE:
Hyalella azteca	8			8					132		40	8		12
ISOPODA:
ASELLIDAE:
Caecidotea sp.										36	36	44	32	4
OSTRACODA:	24	32	4							20
INSECTA:
EPHEMEROPTERA:
BAETIDAE:
Acentrella										10
Callibaetis
CAENIDAE:
Caenis	24	16	24			8						4						16			4
EPHEMERIDAE:
Ephemera simulans
Hexagenia limbata	8		12
HEPTAGENIIDAE:
Stenacron interpunctatum
Stenonema femoratum
ODONATA:
COENAGRIONIDAE:
Enallagma			4		8
CORDULIIDAE:
Epicordulia princeps
GOMPHIDAE:
Gomphus sp.	24	8
HEMIPTERA:
BELOSTOMATIDAE:
Belostoma fuminea
MEGALOPTERA:
SIALIDAE:
Sialis
TRICHOPTERA :
DIPSEUDOPSIDAE:
Phylocentropus		8								4
HELICOPYCHIDAE:
Helicopsyche borealis
HYDROPSYCHIDAE:
Hydropsyche sparna
HYDROPTILIDAE:
Hydroptila
Oxyethira
LEPTOCERIDAE:
Ceraclea
Mystacides sepulchralis
Nectopsyche											4
Oecetis	8
LIMNEPHILIDAE:(early instar)										4
MOLANNIDAE:
Molanna
POLYCENTROPODIDAE:
Polycentropus
LEPIDOPTERA:
PYRALIDAE:
Acentria
COLEOPTERA:
ELMIDAE:
Dubiraphia	64	24	12		8					8	8	16								4	8
Dubiraphia vittata			8
Stenelmis
HALIPLIDAE:
Haliplus
HYDROPHILIDAE:
Berosus
PSEPHENIDAE:
Ectopria nervosa
DIPTERA:
CERATOPOGONIDAE:	24	72	44	8	80	64	32	24	48	12	8	12		4			8		4	4	16
CHAOBORIDAE:
Chaoborus albatus																16	16	72
Chaoborus punctipennis													24				40
CHIRONOMIDAE:
CHIRONOMIDAE:
Chironomus									16						4
Cladopelma									16										4
Cladotanytarsus		24
Cryptochironomus	8	8						8	16			4							8	16	12
Cryptotendipes
Dicrotendipes	32	32					16			32	12	4						16	4	4	4
Endochironomus
Glyptotendipes
Microtendipes			4			8		8	8						12
Nilothauma babiyi	8																				4
Pagastiella ostansa		8		40		6
Parachironomus
Paracladopelma																					4
Paratanytarsus																			16	4	12
Paratendipes
Polypedilum	32	80	8				104	64	72	16	4			16					16	12	24
Pseudochironomus	16		4																	4	4
Stelechomyia perpulchra
Stempellinella			4	72	248	152													4		4
Stenochironomus																					4
Tanytarsus	232	240	76	48	56	16			8	88	20	32	8		4				76	44	136
Tribelos										28	4	8	8	8	4						8
Zavreliella							8			52	8	8	8	12
ORTHOCLADIINAC:
Corynoneura																					4
Cricotopus	8			24	8	16				4	4			4					4
Epoicocladius
Nanocladius	8																			8
Psectrocladius			8
Thienemanniella					8
TANYPODINAE:
Ablabesmyia	40	16	4	40	64	40				60	8			8	8		8				4
Coelotanypus	24		12
Conchapelopia			4
Labrundinia
Paramerina																					8
Procladius	80	64	24	48	32	56		8		4				4	4				8	4	36
TABANIDAE:
Chrysops
TIPULIDAE:
Oromosia (?)				8		16		8	8		4				8
Pseudolimnophila
TOTAL TAXA	26	20	24	17	18	20	12	14	17	26	22	14	13	13	13	1	5	4	15	18	24
TOTAL NUMBERS	792	768	320	528	840	624	800	512	728	660	296	204	200	84	80	16	88	112	600	652	948

Benthic Invertebrate Abundance Data for Lake Sites, Moira River System, Spring 1999 (continued)

Waterbody	Moira R.			Moira R.			Moira R.			Moira R.			Moira R.			Moira R.			Moira R.
TAXON      Site	MR-3	MR-3	MR-3	MR-5	MR-5	MR-5	MR-6	MR-6	MR-6	MR-7	MR-7	MR-7	MR-8	MR-8	MR-8	MR-9	MR-9	MR-9	MR-10	MR-10	MR-10
Replicate	A	B	C	A	B	C	A	B	C	A	B	C	A	B	C	A	B	C	A	B	C
COELENTERATA:
HYDRIDAE:
Hydra										16
PLATYHELMINTHES:
PLANARIIDAE:													4		8
"microturbellaria"												4
NEMERTEA:
Prostoma
NEMATODA:	8	8		16		8	8	8					8		4			32		16
ANNELIDA:
HIRUDINEA:
ERPOBDELLIDAE:
Mooreobdella phalera
GLOSSIPHONIIDAE:
Deserobdella phalera
Helobdella elongata
OLIGOCHAETA:
ENCHYTRAEIDAE:	8									8	4
LUMBRICULIDAE:
Lumbriculus variegatus			8
NAIDIDAE:
Arcteonais lomondi
Dero digitata			8			8													32
Haemonais waldvogeli
Nais communis	8							8						8
Nais variabilis		16
Ophidonais serpentina																				48
Pristina aequiseta										8
Slavinia appendiculata	8
Stylaria lacustris
SPARGANOPHILIDAE:
Sparganophilus eiseni
TUBIFICIDAE:
Aulodrilus americanus			16			4
Aulodrilus limnobius				8
Aulodrilus pluriseta								32		24	2	16	60	80	68		32	32			16
Ilyodrilus templetoni	8	8		8	12	16
Limnodrilus spp.				136	80	36	168	256	176	64		20	84	96	84						16
Limnodrilus claparedianus
Limnodrilus hoffmeisteri										16	6
MOLLUSCA:
BIVALVIA:
SPHAERIIDAE:
Pisidium	40	8		16	4	12	64	64	28	32	18	20	40	48	20	56	16	96		16
Sphaerium striatinum		8	24
GASTROPODA:
ANCYLIDAE:
Ferrissia
HYDROBIIDAE:
Amnicola limosa				8	12	12			8	16	8		272	744	168			16
Amnicola walkeri															12
Probythinella lacustris											2
LYMNAEIDAE: (juvenile)											6			8
Pseudosuccinea columella
PHYSIDAE:
Physella gyrina										24	4	8	4
PLANORBIDAE:
Gyraulus deflectus
Gyraulus parvus										64	12	20	20
Helisoma anceps						8					2
VALVATIDAE:
Valvata tricarinata					4	8				40	8		64	416	40
VIVPARIDAE:
Campeloma decisum		8
Viviparus georgianus		8	16
ACARINA:					12	8		8		8					4		4	8
CRUSTACEA:
AMPHIPODA:
CRANGONYCTIDAE:
Crangonyx
HYALELLIDAE:
Hyalella azteca	80		56			4				16	14	12		8						16
ISOPODA:	80		56			4				16	14	12		8						16
ASELLIDAE:
Caecidotea sp.
OSTRACODA:			8		8	16				24			12					16		16
INSECTA:
EPHEMEROPTERA:
BAETIDAE:
Acentrella
Callibaetis
CAENIDAE:
Caenis				16	8	8	24	32	12	56	28	12	64	104	84	48		32
EPHEMERIDAE:
Ephemera simulans	8
Hexagenia limbata		8			4		8	8		8			4		8	8
HEPTAGENIIDAE:
Stenacron interpunctatum													8
Stenonema femoratum																8
ODONATA:
COENAGRIONIDAE:
Enallagma						4							4		12
CORDULIIDAE:
Epicordulia princeps					4
GOMPHIDAE:
Gomphus sp.			8
HEMIPTERA:
BELOSTOMATIDAE:
Belostoma fuminea											2
MEGALOPTERA:
SIALIDAE:
Sialis						4
TRICHOPTERA :
DIPSEUDOPSIDAE:
Phylocentropus								16	4			4	4					16
HELICOPYCHIDAE:
Helicopsyche borealis															4
HYDROPSYCHIDAE:
Hydropsyche sparna						4
HYDROPTILIDAE:
Hydroptila										24	2	4
Oxyethira						8				24
LEPTOCERIDAE:
Ceraclea															8
Mystacides sepulchralis		8								16	2			32							16
Nectopsyche
Oecetis				8	4					40		8	16		20
LIMNEPHILIDAE:(early instar)
MOLANNIDAE:
Molanna											2
POLYCENTROPODIDAE:
Polycentropus							8
LEPIDOPTERA:
PYRALIDAE:
Acentria
COLEOPTERA:
ELMIDAE:
Dubiraphia	8		24		4	36	24	40	24	8	2		24	32	16			16		32
Dubiraphia vittata
Stenelmis						8				8
HALIPLIDAE:
Haliplus												4
HYDROPHILIDAE:
Berosus														8
PSEPHENIDAE:
Ectopria nervosa															4
DIPTERA:
CERATOPOGONIDAE:	8	48	8	40	32	16	32	48	24	40	4	8	64	120	36	8	16			32	16
CHAOBORIDAE:
Chaoborus albatus
Chaoborus punctipennis																			16		16
CHIRONOMIDAE:
CHIRONOMIDAE:
Chironomus																				16
Cladopelma								16													16
Cladotanytarsus											2	12	8		8	32
Cryptochironomus		8		16	16		16	8	12	16	2	16	8	24
Cryptotendipes	88	72	40
Dicrotendipes			8	168	188	108	40	96	56	192	28	48	32	160	28	16	16	16	32	16
Endochironomus																			16
Glyptotendipes																				16	32
Microtendipes	8	8			4		8		4	16	2
Nilothauma babiyi												4
Pagastiella ostansa					4					8			4
Parachironomus														8
Paracladopelma
Paratanytarsus							56	40	8	64	6	4
Paratendipes																				32
Polypedilum	8	16		16	28	16	32	16	8	16			8	24		16		32
Pseudochironomus					8															16
Stelechomyia perpulchra															4
Stempellinella		8	40			16	8	8		16			4	16	24	32	16	32
Stenochironomus
Tanytarsus	296	368	904	32	48	36	264	128	84	272	34	68	56	16	40	8		16	16
Tribelos							48	72	28							24		32
Zavreliella																				16
ORTHOCLADIINAC:
Corynoneura														8	8
Cricotopus						4	40	40	24	48	14	28	24	16	40
Epoicocladius	8						16
Nanocladius				8	12				8	64		12	8	32	8	8
Psectrocladius						4	24	56	12		4	4			4
Thienemanniella					4			8								8
TANYPODINAE:
Ablabesmyia	8	8				8	8	8	8				4	8		8			32	32
Coelotanypus						12				8							16
Conchapelopia		8	24				16	48	12	72	6	8	32	72	40	8
Labrundinia											2		12
Paramerina				8	12	12															8
Procladius	48	24		24	72	24	32	16	12	24			12	8		40	16	16	48	34	16
TABANIDAE:
Chrysops	8				4
TIPULIDAE:
Oromosia (?)
Pseudolimnophila	8
TOTAL TAXA	19	19	15	16	25	30	22	25	20	35	29	23	32	25	28	17	7	13	7	16	8
TOTAL NUMBERS	664	648	1192	528	588	468	944	1080	552	1392	228	334	972	2096	804	336	128	384	192	368	144

Benthic Invertebrate Abundance Data for Lake Sites, Moira River System, Spring 1999

Waterbody	Moira R			Moira R			Moira R			Moira R
TAXON         Site	MR-12	MR-12	MR-12	MR-13	MR-13	MR-13	MR-14	MR-14	MR-14	MR-15	MR-15	MR-15
Replicate	A	B	C	A	B	C	A	B	C	A	B	C
COELENTERATA:
HYDRIDAE:
Hydra				48	80	16	12	4
PLATYHELMINTHES:
PLANARIIDAE:					16	16	4
"microturbellaria"
NEMERTEA:
Prostoma										8
NEMATODA:	32	16					16	4	8
ANNELIDA:
HIRUDINEA:
ERPOBDELLIDAE:
Mooreobdella fervida						16
GLOSSIPHONIIDAE:
Deserobdella phalera					8
Helobdella elongata
OLIGOCHAETA:
ENCHYTRAEIDAE:				16	32		4
LUMBRICULIDAE:
Lumbriculus variegatus
NAIDIDAE:
Arcteonais lomondi
Dero digitata				48	24	16	36	8	20
Haemonais waldvogeli									4
Nais communis
Nais variabilis
Ophidonais serpentina
Pristina aequiseta				16	8
Slavinia appendiculata				8	8	16	4					8
Stylaria lacustris							4	4	4
SPARGANOPHILIDAE:
Sparganophilus eiseni
TUBIFICIDAE:
Aulodrilus americanus
Aulodrilus limnobius
Aulodrilus pluriseta	64	48	64	8	16		28	48	12
Ilyodrilus templetoni	16
Limnodrilus spp.	80			8	16	32	12	40	76	24	24	40
Limnodrilus claparedianus	16						16		40
Limnodrilus hoffmeisteri	48			8	8		4	4	56
MOLLUSCA:
BIVALVIA:
SPHAERIIDAE:
Pisidium	16	32	16	8	24		12	12	24		32	8
Sphaerium striatinum
GASTROPODA:
ANCYLIDAE:
Ferrissia
HYDROBIIDAE:
Amnicola limosa		16		40	56	160	64	24	72	8	24	40
Amnicola walkeri
Probythinella lacustris
LYMNAEIDAE: (juvenile)
Pseudosuccinea columella					16
PHYSIDAE:
Physella gyrina					24		4	8
PLANORBIDAE:
Gyraulus deflectus									4
Gyraulus parvus				24	24		4		20	16	16	24
Helisoma anceps				8
VALVATIDAE:
Valvata tricarinata							4		12			8
VIVPARIDAE:
Campeloma decisum
Viviparus georgianus				8	24		16	16	28
ACARINA:										8		8
CRUSTACEA:
AMPHIPODA:
CRANGONYCTIDAE:
Crangonyx				16	8
HYALELLIDAE:
Hyalella azteca				32	120	144	20	12	4	112	8	216
ISOPODA:
ASELLIDAE:
Caecidotea sp.				8	32	16				24		16
OSTRACODA:								4
INSECTA:
EPHEMEROPTERA:
BAETIDAE:
Acentrella
Callibaetis										8
CAENIDAE:
Caenis				16	8				8
EPHEMERIDAE:
Ephemera simulans
Hexagenia limbata
HEPTAGENIIDAE:
Stenacron interpunctatum
Stenonema femoratum
ODONATA:
CONENAGRIONIDAE:
Enallagma									4
CORDULIIDAE:
Epicordulia princeps
GOMPHIDAE:
Gomphus sp.
HEMIPTERA:
BELOSTOMATIDAE:
Belastoma fluminea
MEGALOPTERA:
SIALIDAE:
Sialis
TRICHOPTERA:
DIPSEUDOPSIDAE:
Phylocentropus
HELICOPSYCHIDAE:
Helicopsyche borealis
HYDROPSYCHIDAE:
Hydropsyche borealis
HYDROPSYCHIDAE:
Hydropsyche sparna
HYDROPTILIDAE:
Hydroptila									4
Oxyethira									4			8
LEPTOCERIDAE:
Ceraclea
Mystacides sepulchralis
Nectopsyche
Oecetis
LIMNEPHILIDAE: (early instar)
MOLANNIDAE:
Molanna
POLYCENTROPODIDAE:
Polycentropus				16	8					8		8
LEPIDOPTERA:
PYRALIDAE:
Acentria					8
COLEOPTERA:
ELMIDAE:
Dubiraphia				8								32
Dubirapohia vittata
Stenelmis
HALIPLIDAE:
Haliplus
HYDROPHILIDAE:
Berosus
PSEPHENIDAE:
Ectopria nervosa
DIPTERA:
CERATOPOGONIDAE:	32		16	8	16			8	12		8	32
CHAOBORIDAE:
Chaoborus albatus
Chaoborus punctipennis	16
CHIRONOMIDAE:
CHIRONOMINAE:
Chironomus	32	16
Cladopelma
Cladotanytarsus
Cryptochironomus
Cryptotendipes
Dicrotendipes			16	48	16	16	8	12	32	88	16	56
Endochironomus
Glyptotendipes	32	16		16
Microtendipes
Nilothavuma bobiyi
Pagastiella ostansa
Parachironomus
Paracladopelma
Parafanytarsus				8	16		4	48	40
Paratendipes										8		40
Polypedilum				48	104		48	124	4	24		24
Pseudochironomus				56	32			4
Stelechonryia perpulchra
Stempellinella
Stenochironomus
Tanytarsus		16	16		24		40	8	16	24	24	152
Tribelas								4	4
Zavreliella				16								8
ORTHOCLADIINAE:
Corynoneura										8		8
Cricotopus					8		4	16	32	8		56
Epoicocladius
Nanocladius
Psectrocladius							12			16
Thienemanniella												8
TANYPODINAE:
Ablabesmyia				24	8	96		12	8	152		16
Coelotanypus	16
Conchapelopia				16	40	64			4
Labrundinia												24
Paramerina
Procladius	48		16				4				56	16
TABANIDAE:
Chrysops
TIPULIDAE:
Oromosia (?)
Pseudolimnophila
TOTAL TAXA	13	7	6	28	31	12	25	22	28	17	9	24
TOTAL NUMBERS	448	160	144	584	832	608	384	424	556	544	208	856

Benthic Invertebrate Abundance Data for Lake Sites, Moira River System, Spring 1999

Waterbody	Consecon L.			Consecon L.			Consecon L.			Consecon L.			Round L.			Round L.
TAXON      Site	CL-1	CL-1	CL-1	CL-2	CL-2	CL-2	CL-3	CL-3	CL-3	CL-D	CL-D	CL-D	RL-1	RL-1	RL-1	RL-2	RL-2	RL-2
Replicate	A	B	C	A	B	C	A	B	C	A	B	C	A	B	C	A	B	C
PLATYHELMINTHES:
PLANARIIDAE:
NEMERTEA:
Prostoma																	22	38
NEMERTEA:						32								8		2	2	8
ANNELIDA:
HIRUDINEA:
GLOSSIPHONIIDAE:
Helobdella stagnalis
OLIGOCHAETA:
ENCHYTRAEIDAE:
NAIDIDAE:
Arcteonais lomondi																14	22	38
Dero digitata																2	3
Stavinia appendiculata
TUBIFICIDAE:
Aulodrilus americanus							2	10
Aulodrilus limnobius
Aulodrilus pigueti																12	20	22
Aulodrilus pluriseta
Ilyodrilus templetoni										124	76	232				15	14	34
Limnodrilus spp.	16	4	4					2		24	24	76
Limnodrilus claparedianus											4
Limnodrilus hoffmeisteri	8	4								12	4	24
Tubifex tubifex
MOLLUSCA:
BIVALVIA:
SPHAERIIDAE:
Pisidium	4		4													47	54	52
Sphaerium striatinum
UNIONIDAE: (juvenile)
Elliptio complanata
GASTROPODA:
HYDROBILLDAE:
Amnicola limosa								2
VALVATIDAE:
Valvata sincera																2
Valvata tricarinata													4
VIVPARIDAE:
Viviparus georgianus		8						4	6
ACARINA:		4				12			2							7	5	16
CRUSTACEA:
AMPHIPODA:
CRANGONYCTIDAE:
Crangonyx							4
HYALELLIDAE:
Hyalella azteca	20	28	32	2				6					12		4
ISOPODA:
ASELLIDAE:
Caecidotea sp.	16	4
OSTRACODA:						4
INSECTA:
EPHEMEROPTERA:
CAENIDAE:
Caenis	8		4
EPHEMERIDAE
Hexagenia limbata	12	4		6		8	20	24	8
ODONATA:
COENAGRIONIDAE:
Enallagma		4
CORDULIIDAE:
Epicordulia princeps
MEGALOPTERA:
SIALIDAE:
Sialis								2
TRICHOPTERA:
DIPSEUDOPSIDAE:
Phylocentropus									6
HYDROPTILIADAE:
Oxyethira		4
LEPTOCERIDAE:
Leptocerus americamus
POLYCENTROPODIDAE:
Polycentropus		4
DIPTERA:
CERATOPOGONIDAE:							2	6	8				4	8	4
CHAOBORIDAE:
Chaoborus albatus													32	32		147	64	48
Chaoborus flavicans		4								16	24
Chaoborus punctipennis		4	4	20	46	28	2			108	68	112	4			260	41	256
CHIRONOMIDAE:
CHIRONOMINAE:
Chironomus	24	64	8	2	4	20			4	116	64	148	44	8		66	45	64
Cladopelma																1
Cladotanytarsus
Cryptochironomus	16
Dicrotendipes	32	4	12										20	80	20	1
Einfeldia
Endochironomus
Glyptotendipes		24
Microtendipes								2
Parachironomus		136	4						2
Paratanytarsus	40	92
Phaenopsectra flavipes	4
Polypedilum	28	40					8	22	18
Tanytarsus	64	36	60	4	10	4	40	54	38				8				1
Tribelos	16			2					2
Zavreliella
DIAMESINAE:
Monodiamesa							2	2
ORTHOCLADIINAE:
Cricotopus	8	8
Epoicoladius							2		2
Heterotrissocladius		4	4						4
Nanocladius													4
Parakiefferiella															4
Psectrocladius	8	104			2		2						4
Synorthocladius
Thienemanniella
TANYPODINAE:
Ablabesmyia	20				2	4	6	12	12
Coelotanypus		4		2		4	12	18	24
Labrundinia								2
Procladius	12	16	16	22	40	60	8	22	42	12	8	8		8	8	3		12
TOTAL TAXA	19	24	11	8	6	10	13	16	15	7	8	6	11	6	6	14	12	11
TOTAL NUMBERS	356	608	152	60	104	176	110	190	178	412	272	600	140	144	48	579	293	588

Benthic Invertebrate Abundance Data For Lake Sites, Moira River System, Spring 1999

Waterbody	Round L.			Moira River (Bend Bay)			Moira L.			Moira L.			Moira L.			Moira L.			Moira L.
Taxon           Site	RL-3	RL-3	RL-3	MR-11	MR-11	MR-11	ML-1	ML-1	ML-1	ML-2	ML-2	ML-2	ML-3	ML-3	ML-3	ML-4	ML-4	ML-4	ML-5	ML-5	ML-5
Replicate	A	B	C	A	B	C	A	B	C	A	B	C	A	B	C	A	B	C	A	B	C
PLATYHELMINTHES:
PLANARIDAE:	2	1				8
NEMERTEA:
Prostoma		2														8	80	48	8		8
NEMATODA:	28	41	14		4		76	60	96	8	4	26
ANNELIDA:
HIRUDINEA:
GLOSSIPHONIIDAE:
Helobdella stagnalis	10	2	3
OLIGOCHAETA:
ENCHYTRAEIDAE:
NAIDIDAE:
Arcteonais lomondi	14	12	3
Dero digitata	12	17	6	140	128	96	52	16	52	14	8	4
Slavinia appendiculata
TUBIFICIDAE:
Aulodrilus americanus
Aulodrilus limnobius																				8
Aulodrilus pigueti	2			12		16
Aulodrilus pluriseta				8																	24
llyodrilus templetoni	2						24	10		12	12	20		32		56	48	24
Limnodrilus spp.			3				36	24	96				24	8		8	16	16	16	8	8
Limnodrilus claparedianus
Limnodrilus hoffmeisteri																				16	8
Tubifex tubifex
MOLLUSCA:
BIVALVIA:
SPHAERIIDAE:
Pisidium	160	111	123	4	20		24	8	36	4	4	2							32	40	16
Sphaerium striatinum
UNIONIDAE:(juvenile)
Elliptio complanata
GASTROPODA:
HYDROBIIDAE:
Amnicola limosa	2	3	3					4
VALVATIDAE:
Valvata sincera	10	10	5
Valvata tricarinata
VIVPARIDAE:
Viviparus georgianus
ACARINA:	6								8			2			8
CRUSTACEA:
AMPHIPODA:
CRANGONYCTIDAE:
Crangonyx	2
HYALELLIDAE:
Hyalella azteca
ISOPODA:
ASELLIDAE:
Caecidotea sp.
OSTRACODA:				8	20	16		2			8
INSECTA:
EPHEMEROPTERA:
CAENIDAE:
Caenis	2		1
EPHEMERIDAE:
Hexagenia limbata
ODONATA:
COENAGRIONIDAE:
Enallagma
CORDULIIDAE:
Epicordulia princeps
MEGALOPTERA:
SIALIDAE:
Sialis
TRICHOPTERA:
DIPSEUDOPSIDAE:
Phylocentropus
HYDROPTILIDAE:
Oxyethira
LEPTOCERIDAE:
Leptocerus americanus
POLYCENTROPODIDAE:
Polycentropus
DIPTERA:
CERATOPOGONIDAE:			1		8		16	16	20			2							8	8	16
CHAOBORIDAE:
Chaoborus albatus	48	48	17	84	80	80	40	16	72	46	42	30	112	144	48	8	16
Chaoborus flavicans
Chaoborus punctipennis	52	56	12	72	40	72	20	34	40	28	34	36	64	56	64	24		8	16	24	16
CHIRONOMIDAE:
CHIRONOMINAE:
Chironomus	20	32	38					2			2				16					8	16
Cladopelma	2		1		16		16	8	24	4	2										8
Cladotanytarsus		1
Cryptochironomus			2					2	4			2
Dicrotendipes	2	2		8		8														8
Einfeldia
Endochironomus
Glyptotendipes
Microtendipes
Parachironomus
Paratanytarsus
Phaenopsectra flavipes
Polypedilum				4
Tanytarsus	6	2	3				4											16		24
Tribelos			4
Zavreliella
DIAMESINAE:
Monodiamesa
ORTHOCLADIINAE:
Cricotopus											2	2
Epoicocladius
Heterotrissocladius
Nanocladius
Parakiefferiella
Psectrocladius
Synorthocladius
Thienemanniella
TANYPODINAE:
Ablabesmyia				4						2									8
Coelotanypus
Labrundinia
Procladius	16	25	16	44	48	32	20	10	28	22	30	38	32	56	72	24	24	80	16	58	32
TOTAL TAXA	20	16	18	11	9	8	11	13	12	9	12	12	4	5	5	6	5	6	7	10	10
TOTAL NUMBERS	398	365	255	388	364	328	328	208	480	140	152	174	232	296	208	128	184	192	104	200	152

Benthic Invertebrate Abundance Data for Lake Sites, Moira River System, Spring 1999

Waterbody	Stoco L.			Stoco L.			Stoco L.			Stoco L.			Stoco L.
Taxon      Site	SL-1	SL-1	SL-1	SL-2	SL-2	SL-2	SL-3	SL-3	SL-3	SL-4	SL-4	SL-4	SL-5	SL-5	SL-5
Replicate	A	B	C	A	B	C	A	B	C	A	B	C	A	B	C
PLATYHELMINTHES:
PLANARIDAE:							4		2
NEMERTEA:
Prostoma
NEMATODA:		8	8					4	1			1	1	1
ANNELIDA:
HIRUDINEA:
GLOSSIPHONIIDAE:
Helobdella stagnalis
OLIGOCHAETA:
ENCHYTRAEIDAE:									2
NAIDIDAE:
Arcteonais lomondi
Dero digitata				2	6	5			14	5		10	35	34	13
Slavinia appendiculata													1
TUBIFICIDAE:
Aulodrilus americanus
Aulodrilus limnobius
Aulodrilus pigueti													15	16	9
Aulodrilus pluriseta		32	32
Ilyodrilus templetoni
Limnodrilus spp.		24	8	1	1	1	10	8	10	14	14	12	1
Limnodrilus claparedianus
Limnodrilus hoffmeisteri			8				8	6	4		6	1
Tubifex tubifex	8	24	8	3	8	17	10	4	29	21	8	18	3	2	2
MOLLUSCA:
BIVALVIA:
SPHAERIIDAE:
Pisidium			16	10	11	7	26	18	17	22	12	17	8	4	4
Sphaerium striatinum														1
UNIONIDAE:(JUVENILE)									1
Elliptio complanata								2
GASTROPODA:
HYDROBIIDAE:
Amnicola limosa	8	40	8	5	5	4	84	34	34
VALVATIDAE:
Valvata sincera		8		5	3	4
Valvata tricarinata				5	11	5	18	2	7
VIVPARIDAE:
Viviparus georgianus
ACARINA:					1	3							1
CRUSTACEA:
AMPHIPODA:
CRANGONYCTIDAE:
Crangonyx
HYALELLIDAE:
Hyalella azteca							4	4	2				1
ISOPODA:
ASELLIDAE:
Caecidotea sp.
OSTRACODA:										1
INSECTA:
EPHEMEROPTERA:
CAENIDAE:
Caenis
EPHEMERIDAE:
Hexagenia limbata
ODONATA:
COENAGRIONIDAE:
Enallagma
CORDULIIDAE:
Epicordulia princeps							2
MEGALOPTERA:
SIALIDAE:
Sialis				2	3	4	2		1						1
TRICHOPTERA:
DIPSEUDOPSIDAE:
Phylocentropus
HYDROPTILIDAE:
Oxyethira
LEPTOCERIDAE:
Leptocerus americanus	8	16	24			1
POLYCENTROPODIAE:
Polycentropus
DIPTERA:
CERATOPOGONIDAE:						1	2			5	10	6	4	4	4
CHAOBORIDAE:
Chaoborus albatus				5	5	2		2		25	51	54	43	29	31
Chaoborus flavicans
Chaoborus punctipennis			24	3	2	7	4	2		57	70	50	29	30	24
CHIRONOMIDAE:
CHIRONOMINAE:
Chironomus		24				2	2		1	1	1	1
Cladopelma					1		4		3	2	1			1	1
Cladotanytarsus
Cryptochironomus		8					2	2	2
Dicrotendipes							14	6	3	2		1	1
Einfeldia					5	4	2
Endochironomus											1
Glyptotendipes
Microtendipes				1
Parachironomus
Paratanytarsus							2		1
Phaenopsectra flavipes
Polypedihim	8		8				22	16	16
Tanytarsus			8	4	2	6	4		2			1	2
Tribelos		8					12		4
Zavreliella	8
DIAMESINE:
Monodiamesa
ORTHOCLADIINAE:
Cricotopus
Epoicocladius												1
Heterotrissocladius
Nanocladius
Parakiefferiella
Psectrocladius
Synorthocladius		16
Thienemanniella														1
TANYPODINAE:
Ablabesmyia				1				2	1
Coelotonypus
Labrundinia
Procladius	56	48	24	27	84	74	34	14	11	67	35	70	42	31	28
TOTAL TAXA	6	12	12	14	15	17	22	16	23	12	11	14	15	12	10
TOTAL NUMBERS	96	256	176	74	148	147	272	126	168	222	209	243	187	154	117

Appendix III.2           Results of Toxicity Tests Using Chironomus riparius and Hyalella azteca

29 July 1999

Mr. Jim Kroetch
Golder Associates
2180 Meadowvale Blvd.
Mississauga, ON
L5N5S3

Dear Mr. Kroetch,

Re: Toxicity Analysis of Sediment Samples

Please find the enclosed reports for sediment toxicity testing conducted on samples received May 10, 1999. The reports include both the results and data regarding the test conditions.

As reported previously, tests conducted using Chironomus riparius  and initiated on June 8, 1999 were invalid because control mortality exceeded the maximum allowed under Environment Canada Protocols. As required under internal quality control practices, and investigation of the test system was initiated to determine if the cause of the control mortality could be identified. Our investigation included a review of internal procedures related to the performance of the test systems during the period. They included but were not limited to light intensity and photoperiod maintenance, temperature, feeding regime, aeration rates, preparation of test chambers, routine chemistry of control water, organism history and handling. No abnormalities were identified with respect to the operation of the test system. There was no unusual mortality observed in reference toxicant testing conducted within a week of the test. It must be noted however, that reference toxicant assays for sediment tests differ slightly from the actual sediment test in that they are shorter term and test performance of the organism is in a "water only" environment.

Remedial action included immediately informing the test sponsor, Golder Associates. Approval was given for a retest of the samples. The retest was initiated on June 23, 1999. Test reports for these sediments indicate that storage time exceeded 6 weeks.

Mr. Jim Kroetch
July 29, 1999

---

As stated in our original proposal, leftover sediments are to be returned to the client for disposal. We await your shipping instructions.

Sincerely,
ESG INTERNATIONAL INC.

Jim Reid
Laboratory Supervisor

Enclosures

JR/jr

SEDIMENT TEST REPORT
Hyalella azteca Survival and Growth
1 of 5

Sample Identification
Company :	Golder Associates	Date Tested	05/21/99
Location :	Missassauga, ON	Data Test Completed :	06/04/99
Sample Method :	Grab	Shipped by:	R. Yaagumagi
Sampled By :	R.Yaagumagi	Lab Storage (° C):	6.0
Date and Time Received :	05/10/99@ 11 00	Sample Volume :	10 L

Substance	Date Collected	ESG Sample Number	Description
Control			-fine black sediment, no strong odour.
Stoco LK. SL-1	04/22/99	03990548	-fine dark olive-brown sediment, some organic debris, slight odour,
Stock LK. SL-3	04/22/99	03990562	-fine, dark olive-brown sediment, lots of organic debris, slight odour.
Stock LK. SL-4	04/22/99	03990563	-fine, dark olive-brown sediment, slight odour.
Round LK. RL-1	04/23/99	03990558	-fine, dark olive-brown sediment, some plant material, slight odour.
Round LK. RL-2	04/23/99	03990559	-fine dark brown-green sediment, no strong odour.
Round LK. RL-3	04/23/99	03990560	-fine, dark brown-black sediment, no strong odour.

Results

Hvalella azteca Survival

Hyalella azteca Growth

Station	Replicate	%Surviving Organisms	Overall Average Survival %	SD	CV	Mean Dry wt/organism (mg)	Overall Average wt/Organism (mg)	S.D	CV
	A	100	92	4.472	4.861	0.143	0.124	0.012	9.555
	B	90				0.118
control	C	90				0.127
	D	90				0.118
	E	90				0.113
	A	100	96	8.944	9.317	0.148	0.146	0.022	14.714
	B	100				0.123
SL-1	C	100				0.131
03990548	D	80				0.179
	E	100				0.151
	A	100	100	0	0	0.129	0.145	0.014	9.536
	B	100				0.156
SL-3	C	100				0.159
03990562	D	100				0.132
	E	100				0.150
	A	100	98	4.472	4.563	0.130	0.149	0.015	10.061
	B	90				0.147
SL-4	C	100				0.172
03990563	D	100				0.151
	E	100				0.147
	A	80	86	8.944	10.400	0.133	0.152	0.023	15.225
	B	80				0.141
RL-1	C	90				0.133
03990558	D	80				0.184
	E	100				0.169
	A	90	96	5.477	5.705	0.124	0.124	0.011	8.739
	B	100				0.108
RL-2	C	90				0.121
03990558	D	100				0.136
	E	100				0.132
	A	90	92	4.472	4.861	0.108	0.110	0.009	7.716
	B	90				0.107
RL-3	C	100				0.115
03990560	D	90				0.122
	E	90				0.100

Technician:_____________________

Toxicity Test Chemistry Parameters
					Day
Sediment		0	3	5	7	10	12	14
Control	Temp. (°C)	24.0	24.0	23.5	23.0	23.0	23.0	23.0
	D.O. (mg/L)	8.0	8.0	6.9	7.9	8.0	8.2	8.0
	pH	8.4						8.5
	Conductivity	546						676
	Ammonia (mg/L)	2.6						0.25
	Hardness (mg/L)	270						320
Stoco Lk.-SL-1	Temp. (°C)	24.0	24.0	23.5	23.0	23.0	23.0	23.0
03990548	D.O. (mg/L)	7.7	7.7	6.7	8.2	7.5	8.2	8.4
	pH	8.0						8.2
	Conductivity	431						356
	Ammonia (mg/L)	1.25						0.25
	Hardness (mg/L)	220						160
Stoco Lk.-SL-3	Temp. (°C)	24.0	24.0	23.5	23.0	23.0	23.0	23.0
03990562	D.O. (mg/L)	8.1	8.2	7.2	8.2	8.0	8.1	8.4
	pH	8.3						8.3
	Conductivity	504						468
	Ammonia (mg/L	2.75						0.25
	Hardness (mg/L)	270						200
Stoco Lk.-SL-4	Temp. (°C)	24.0	24.0	23.5	23.0	23.0	23.0	23.0
03990563	D.O. (mg/L	7.8	8.1	6.8	8.2	7.7	8.2	8.3
	pH	8.1						7.9
	Conductivity	436						298
	Ammonia (mg/L	1.35						0.3
	Hardness (mg/L)	210						140
Round Lk.-RL-1	Temp. (°C)	24.0	24.0	23.5	23.0	23.0	23.0	23.0
03990558	D.O. (mg/L)	7.8	8.2	7.9	8.2	8.2	8.4	8.6
	pH	8.2						8.2
	Conductivity	517						410
	Ammonia (mg/L)	1.25						0.2
	Hardness (mg/L)	240						170
Round Lk.-RL-2	Temp. (°C)	24.0	24.0	23.5	23.0	23.0	23.0	23.0
03990559	D.O. (mg/L)	7.9	8.0	7.3	8.2	8.1	8.3	8.4
	pH	8.1						8.1
	Conductivity	397						356
	Ammonia (mg/L)	0.7						0.1
	Hardness (mg/L)	200						160
Round Lk.-RL-3	Temp. (°C)	24.0	24.0	23.5	23.0	23.0	23.0	23.0
03990560	D.O. (mg/L)	7.8	8.0	6.5	8.4	8.2	8.3	8.4
	pH	7.9						7.8
	Conductivity	406						390
	Ammonia (mg/L)	0.85						0.1
	Hardness (mg/L)	210						170

Date:_____________________

Approved by:_______________________
Jim Reid, Laboratory Supervisor

Test Conditions
Test Organism:	Hyalella azteca	Sediment Volume (mL):	100
Source:	AIB, Burlington, On	Control/Dilution Water:	undiluted well Watera
Life Stage at Start:	2 - 9 day old	Overlying Water Volume (mL):	175
Test Type:	Static	Test Aeration Rate:	2-3bubbles/second
No. Discrete Samples/ Treatment:	1	Photoperikod (h) light/dark:	16/8
Number of Replicates:	5	Temperature:	23 ± 1°C
Number of Organisms per Replicate:	10	Feeding Rate:	2.76 mg dry solids in 1.5 mL suspension / replicate
Number of Organisms per Treatment:	50	Feeding Frequency:	Daily
Sediment Depth:	approx. 3.5 cm	Test Duration:	14 days

a Control/Dilution Water: No chemicals added.

Test Protocol: Biological Test Method: Test for Survival and Growth in Sediment Using the Freshwater amphipod Hyalella azteca. Environment Canada, Conservation and and Protection. Ottawa, Ontario. Report EPS 1/RM/32.

Test Organisms: No organisms exhibiting unusual appearance, behaviour, or undergoing unusual treatment were used in the test. Previous 48 h culture mortality <1%

Control Sediment: Obtained from Long Point, Ontario

Test Conditions: There were no unusual conditions or deviations from the test protocol.

References

^aWest, Inc, and D.Gulley. 1996. Toxstat Release 3.5 Western Ecosystems Technology. Cheyenne, WY, U.S.A

Sample Identification
Company :	Golder Associates	Date Tested	06/02/99
Location :	Missassauga, ON	Data Test Completed :	06/12/99
Sample Method :	Grab	Shipped by:	R. Yaagumagi
Sampled By :	R.Yaagumagi	Lab Storage (° C):	6.0
Date and Time Received :	05/10/99@ 11 00	Sample Volume :	10 L

Substance	Date Collected	ESG Sample Number	Description
Control			-fine black sediment, no strong odour.
Stoco LK. SL-1	04/22/99	03990548	-fine dark olive-brown sediment, some organic debris, slight odour,
Stock LK. SL-3	04/22/99	03990562	-fine, dark olive-brown sediment, lots of organic debris, slight odour.
Stock LK. SL-4	04/22/99	03990563	-fine, dark olive-brown sediment, slight odour.
Round LK. RL-1	04/23/99	03990558	-fine, dark olive-brown sediment, some plant material, slight odour.
Round LK. RL-2	04/23/99	03990559	-fine dark brown-green sediment, no strong odour.
Round LK. RL-3	04/23/99	03990560	-fine, dark brown-black sediment, no strong odour.

Results

Chironomus riparius Survival

Chironomus riparius Growth

Station	Replicate	%Surviving Organisms	Overall Average Survival %	SD	CV	Mean Dry wt/organism (mg)	Overall Average wt/Organism (mg)	S.D	CV
	A	100	92	12.247	13.608	0.623	0.517	0.296	57.338
	B	90				0.489
control	C	70				0.547
	D	90				0.871
	E	100				0.055
	A	100	84	18.166	21.626	0.523	0.617	0.127	20.589
	B	60				0.450
SL-1	C	70				0.646
03990548	D	100				0.179
	E	90				0.746
	A	60	78	21.679	27.794	0.753	0.701	0.205	29.279
	B	50				0.640
SL-3	C	100				0.573
03990562	D	90				1.030
	E	90				0.508
	A	70	80	14.142	17.678	0.859	0.794	0.108	13.640
	B	90				0.727
SL-4	C	90				0.636
03990563	D	90				0.881
	E	60				0.868
	A	100	98	4.472	4.563	0.611	0.553	0.091	16.478
	B	100				0.403
RL-1	C	100				0.529
03990558	D	100				0.618
	E	90				0.603
	A	100	86	16.733	19.457	0.548	0.469	0.085	18.144
	B	90				0.512
RL-2	C	60				0.527
03990558	D	80				0.352
	E	100				0.406
	A	90	74	20.736	28.022	0.439	0.407	0.127	31.145
	B	70				0.586
RL-3	C	80				0.289
03990560	D	40				0.280
	E	90				0.442

Technician:_____________________

Toxicity Test Chemistry Parameters
				Day
Sediment		0	2	5	7	90	10
Control	Temp. (°C)	23.0	22.5	22.5	22.5	23.0	23.0
	D.O. (mg/L)	8.3	8.5	4.8	8.4	8.5	8.3
	pH	8.3					8.1
	Conductivity	451					430
	Ammonia (mg/L)	4.1					0.55
	Hardness (mg/L)	260					210
Stoco Lk.-SL-1	Temp. (°C)	23.0	22.5	22.5	22.5	23.0	23.0
03990548	D.O. (mg/L)	8.3	8.3	8.5	8.3	7.9	6.9
	pH	8.3					7.6
	Conductivity	417					376
	Ammonia (mg/L)	0.9					0.4
	Hardness (mg/L)	270					170
Stoco Lk.-SL-3	Temp. (°C)	23.0	22.5	22.5	22.5	23.0	23.0
03990562	D.O. (mg/L)	8.3	8.0	7.5	8.0	8.3	7.9
	pH	8.4					8.1
	Conductivity	533					438
	Ammonia (mg/L	2.25					0.3
	Hardness (mg/L)	250					220
Stoco Lk.-SL-4	Temp. (°C)	23.0	22.5	22.5	22.5	23.0	23.0
03990563	D.O. (mg/L	8.3	8.0	8.4	7.5	8.6	8.1
	pH	8.0					7.6
	Conductivity	404					363
	Ammonia (mg/L	0.35					0.35
	Hardness (mg/L)	220					160
Round Lk.-RL-1	Temp. (°C)	23.0	22.5	22.5	22.5	23.0	23.0
03990558	D.O. (mg/L)	8.2	8.2	8.4	7.9	7.5	7.6
	pH	8.3					8.0
	Conductivity	405					411
	Ammonia (mg/L)	2.1					2.9
	Hardness (mg/L)	200					180
Round Lk.-RL-2	Temp. (°C)	23.0	22.5	22.5	22.5	23.0	23.0
03990559	D.O. (mg/L)	8.6	8.0	8.4	8.3	8.6	8.3
	pH	8.3					8.0
	Conductivity	378					363
	Ammonia (mg/L)	0.35					180
	Hardness (mg/L)	230					180
Round Lk.-RL-3	Temp. (°C)	23.0	22.5	22.5	22.5	23.0	23.0
03990560	D.O. (mg/L)	8.4	8.4	8.5	8.3	8.5	8.3
	pH	8.2					8.0
	Conductivity	407					418
	Ammonia (mg/L)	0.5					1.95
	Hardness (mg/L)	210					180

Date:_____________________

Approved by:_______________________
Jim Reid, Laboratory Supervisor

Test Conditions
Test Organism	Chironomus riparius	Sediment Volume (mL)	100
Source	University of Waterloo	Control/Dilution Water	undiluted well Watera
Life Stage at Start	1stinstar (<48h post hatch)	Overlying Water Volume (mL)	175
Test Type	Static	Test Aeration Rate	2-3bubbles/second
No. Discrete Samples/ Site	1	Photoperikod (h) light/dark	16/8
Number of Replicates	5	Temperature	23 ± 1°C
Number of Organisms per Replicate	10	Feeding Rate	6 mg dry solids in 1.5 mL suspension / replicate
Number of Organisms per Treatment	50	Feeding Frequency	Daily
Sediment Depth	approx. 3.5 cm	Test Duration	10 days

a Control/Dilution Water: No chemicals added.

Test Protocol: Biological Test Method: Test for Survival and Growth in Sediment Using the larvae of Freshwater Midges (Chironomus tentans or Chironomus riparius). Environment Canada, Conservation and and Protection. Ottawa, Ontario. Report EPS 1/RM/32.

Test Organisms: No organisms exhibiting unusual appearance, behaviour, or undergoing unusual treatment were used in the test.

Control Sediment: Obtained from Long Point, Ontario

Test Conditions: There were no unusual conditions or deviations from the test protocol.

References

^aWest, Inc, and D.Gulley. 1996. Toxstat Release 3.5 Western Ecosystems Technology. Cheyenne, WY, U.S.A

Sample Identification
Company :	Golder Associates	Date Tested	05/26/99
Location :	Missassauga, ON	Data Test Completed :	06/09/99
Sample Method :	Grab	Shipped by:	R. Yaagumagi
Sampled By :	R.Yaagumagi	Lab Storage (° C):	6.0
Date and Time Received :	05/10/99@ 11 00	Sample Volume :	10 L

Substance	Date Collected	ESG Sample Number	Description
Control			-fine black sediment, no strong odour.
Consecon LK. CL-1	04/22/99	03990547	-coarse, greenish-grey sediment, large amounts of organic material (molluse shells), slight odour.
Consecon LK. CL-2	04/26/99	03990546	-very fine, dark olive-green sediment, sediment, slight odour.
Consecon LK. CL-3	04/26/99	03990544	-fine, coarse, dark olive-green sediment, large amounts of organic material (molluses shells, wood), some odour.
Consecon LK. CL-Deep Basin	05/03/99	03990549	-fine, dark green-gray sediment, slight odour.
Moira Lk. ML-1	04/22/99	03990551	-very fine blcak sedimetn,slight odour.
Moira Lk. ML-4	04/27/99	03990557	-fine, black sediment, slight odour.
Moira Lk. ML-5	04/27/99	03990556	-coarse, black sediment, large amounts of organic material (molluse shells), slight odour.

Results

Hyalella aztecaSurvival

Hyalella azteca Growth

Station	Replicate	%Surviving Organisms	Overall Survival%	SD	CV	Mean dry wt. per Organism (mg)	Overall Average wt/Organism (mg)	S.D.	CV
Control	A	100	90	10.000	11.111	0.179	0.182	0.022	12.266
	B	100				0.155
	C	80				0.200
	D	80				0.168
	E	90				0.209
CL-1 03990547	A	100	92	13.038	14.172	0.177	0.174	0.023	13.014
	B	90				0.144
	C	100				0.194
	D	100				0.157
	E	70				0.196
CL-2 03990654	A	100	94	8.944	9.515	1.165	0.145	0.019	13.126
	B	100				0.140
	C	80				0.115
	D	100				0.147
	E	90				0.156
CL-3 03990544	A	100	86	19.494	22.667	0.155	0.179	0.034	19.244
	B	60				0.152
	C	70				0.174
	D	100				0.237
	E	100				0.175
CL-Deep Basin 03990549	A	100	88	10.954	12.448	0.184	0.160	0.027	16.661
	B	80				0.160
	C	80				0.188
	D	100				0.123
	E	80				0.147
ML-1 03990551	A	90	84	8.9442719	10.647943	0.076	0.092	0.014	15.442
	B	90				0.109
	C	90				0.099
	D	70				0.079
	E	80				0.096
ML-4 03990557	A	90	82	4.472	5.454	0.129	0.125	0.008	6.582
	B	80				0.129
	C	80				0.129
	D	80				0.110
	E	80				0.126
ML-5 03990556	A	80	88	10.954	12.448	0.199	0.166	0.027	16.142
	B	80				0.136
	C	100				0.158
	D	80				0.190
	E	100				0.149

Technician:___________________

Toxicity Test Chemistry Parameters

Sediment		0	2	5	Day 7	9	12	14
Control	Temp. (°C)	23.0	23.0	23.0	23.0	22.5	22.5	23.0
	D.O. (mg/L)	8.4	8.3	8.4	8.3	8.2	8.3	8.2
	pH	8.4						8.4
	Conductivity	541						658
	Ammonia (mg/L)	0						0.05
	Hardness (mg/L)	270						340
Consecon Lk.-CL-1 03990547	Temp. (°C)	22.0	23.0	23.0	23.0	22.5	22.5	23.0
	D.O. (mg/L)	8.4	8.4	8.4	8.3	8.7	8.6	8.4
	pH	8.5						8.4
	Conductivity	510						478
	Ammonia (mg/L)	0						0.05
	Hardness (mg/L)	190						240
Consecon Lk.-CL-2 03990546	Temp. (°C)	22.0	23.0	23.0	23.0	22.0	22.5	23.0
	D.O. (mg/L)	8.3	8.5	8.3	8.5	8.5	8.5	7.3
	pH	8.4						8.4
	Conductivity	504						615
	Ammonia (mg/L)	1.15						0.05
	Hardness (mg/L)	260						260
Consecon Lk.-CL-3 03990544	Temp. (°C)	23.0	23.0	23.0	23.0	22.0	22.5	23.0
	D.O. (mg/L)	8.4	8.5	8.6	8.5	8.3	8.5	7.9
	pH	8.5						8.4
	Conductivity	524						561
	Ammonia (mg/L)	0						0.1
	Hardness (mg/L)	320						260
Consecon Lk. CL-Deep Basin 03990549	Temp (°C)	23.0	23.0	23.0	23.0	22.5	22.5	23.0
	D.O. (mg/L)	8.4	8.4	8.0	8.4	8.6	8.4	7.5
	pH	8.5						8.5
	Conductivity	533						674
	Ammonia (mg/L)	0						0.1
	Hardness (mg/L)	280						360
Moira Lk.-ML-1 03990551	Temp. (°C)	22.0	23.0	23.0	23.0	22.5	22.5	23.0
	D.O. (mg/L)	8.6	8.3	8.3	8.1	8.3	8.3	8.1
	pH	8.4						8.3
	Conductivity	495						506
	Ammonia (mg/L)	0						0.05
	Hardness (mg/L)	270						260
Moira Lk.-ML-4 03990557	Temp. (°C)	23.0	23.0	23.0	23.0	22.5	22.5	23.0
	D.O. (mg/L)	8.3	8.5	8.5	8.3	8.3	8.5	8.2
	pH	8.4						8.1
	Conductivity	516						544
	Ammonia (mg/L)	0						0
	Hardness (mg/L)	370						280
Moira Lk.-ML-5 03990556	Temp. (°C)	22.0	23.0	23.0	23.0	22.5	22.5	23.0
	D.O. (mg/L)	8.5	8.4	8.4	8.4	8.5	8.5	8.1
	pH	8.4						8.2
	Conductivity	496						543
	Ammonia (mg/L)	0.0						0.1
	Hardness (mg/L)	260						280

Date:________________________

Approved by:___________________________
Jim Reid, Laboratory Supervisor

Test Conditions

Test Organism	:	Hyalella azteca	Sediment Volume (mL)	:	100
Source	:	AIB, Burlington, ON	Control/Dilution Water	:	undiluted well watera
Life Stage at Start	:	2-9 day old	Overlying Water Volume (mL)	:	175
Test Type	:	Static	Test Aeration Rate	:	2-3 bubbles/second
No. Discrete Samples/Treatment	:	1	Photoperiod (h) light/dark	:	16/8
Number of Replicates	:	5	Temperature	:	23 ± 1 °C
Number of Organisms per Replicate	:	10	Feeding Rate	:	2.7 mg dry solids in 1.5 mL suspension / replicate
Number of Organisms per Treatment	:	50	Feeding Frequency	:	Daily
Sediment Depth	:	3.5 cm	Test Duration	:	14 days

^a Control/Dilution Water:  No chemicals added.

Test Protocol:      Biological Test Method: Test for Survival and Growth in Sediment Using the Larvae of Freshwater Amphipod Hyalella aztec. Environment Canada, Conservation and Protection. Ottawa, Ontario. Report EPS 1/RM/33.

Test Organisms:      No organisms exhibiting unusual appearance, behaviour, or undergoing unusual treatment were used in the test. Previous 48 h culture mortality <1%.

Control Sediment:      Obtained from long Point, Ontario.

Test Conditions:      There were no unusual conditions or deviations from the test protocol.

Reference Toxicant Date

References

^aWest, Inc. and D. Gulley. 1996. Toxstat Release 3.5. Western Ecosystems Technology. Cheyenne, WY, U.S.A

Sample Identification

 

Results

Chironomus riparius Survival

Chironomus riparius Growth

Station	Replicate	%Surviving Organisms	Overall Average % Survival	SD	CV	Mean Dry wt/Organism (mg)	Overall Average wt/organism (mg)	S.D.	CV
Control	A	90	84	8.944	10.648	0.583	0.501	0.067	13.364
	B	90				0.433
	C	70				0.433
	D	80				0.514
	E	90				0.543
CL-1 03990547	A	90	84	18.166	21.626	0.688	0.679	0.169	24.923
	B	100				0.792
	C	70				0.490
	D	60				0.890
	E	100				0.533
CL-2 03990546	A	100	64	29.665	46.351	0.755	0.811	0.282	34.820
	B	60				0.490
	C	60				0.812
	D	20				1.265
	E	80				0.731
CL-3 03990544	A	60	56	40.373	72.095	0.955	0.758	0.365	48.181
	B	10				0.150
	C	100				0.821
	D	90				0.757
	E	20				1.105
CL-Deep Basin 03990549	A	100	94	8.944	9.515	0.682	0.715	0.148	20.701
	B	100				0.733
	C	80				0.901
	D	100				0.543
	E	90				0.497
ML-1 3990551	A	60	74	13.416	18.130	0.375	0.388	0.093	23.885
	B	60				0.297
	C	90				0.444
	D	80				0.515
	E	80				0.307
ML-4 3990557	A	100	78	30.332	38.887	0.577	0.582	0.061	10.561
	B	100				0.621
	C	50				0.508
	D	40				0.662
	E	100				0.541
ML-5 3990556	A	60	56	41.593	74.274	0.398	0.414	0.105	25.483
	B	90				0.563
	C	0				0.000
	D	30				0.317
	E	100				0.376

Technician:_______________

Toxicity Test Chemistry Parameters

				Day
Sediment		0	1	4	6	8	10
Control	Temp.(°C)	23.5	24.0	24.0	23.5	23.0	22.0
	D.O. (mg/L)	7.9	7.8	7.4	8.2	8.1	7.8
	pH	8.2					8.3
	Conductivity	536					599
	Ammonia (mg/L)	2.75					0.7
	Hardness (mg/L)	260					300
Consecon Lk.-CL-1 03990547	Temp. (°C)	23.5	24.0	24.0	23.5	23.0	22.0
	D.O. (mg/L)	8.1	7.9	8.2	7.8	8.0	8.0
	pH	8.2					8.3
	Conductivity	535					494
	Ammonia (mg/L)	0.95					0.5
	Hardness (mg/L	260					230
Consecon Lk.-CL-2 03990546	Temp (°C)	23.5	24.0	24.0	23.5	23.0	22.0
	D.O (mg/L)	8.2	8.1	8.1	7.6	8.0	7.8
	pH	8.3					8.2
	Conductivity	510					520
	Ammonia (mg/L)	0.6					0.6
	Hardness (mg/L)	260					300
Consecon Lk-CL-3 03990544	Temp (°C)	23.5	24.0	24.0	23.5	23.0	22.0
	D.O. (mg/L)	8.3	8.1	8.3	7.5	5.3	5.6
	pH	8.0					8.2
	Conductivity	679					685
	Ammonia (mg/L)	0.65					1.9
	Hardness (mg/L)	300					310
Conseon Lk.-Deep Basin 03990549	Temp (°C)	23.5	24.0	24.0	23.5	23.0	22.0
	D.O.(mg/L)	8.0	8.1	8.1	7.8	8.0	8.1
	pH	8.2					8.2
	Conductivity	524					538
	Ammonia (mg/L)	1.5					0.6
	Hardness (mg/L)	260					270
Moira Lk. -ML-1 03990551	Temp. (° C)	23.5	24.0	24.0	23.5	23.0	22.0
	D.O(mg/L)	7.6	8.1	8.3	7.4	8.2.	8.2
	pH	8.3					8.1
	Conductivity	495					470
	Ammonia (mg/L)	1.02					0.6
	Hardness (mg/L)	260					260
Moira Lk. - ML-4 03990557	Temp. (°C)	23.5	24.0	24.0	23.5	23.0	22.0
	D.O.(mg/L)	8.5	8.3	7.9	7.3	7.9	7.4
	pH	8.4					8.0
	Conductivity	506					496
	Ammonia (mg/L)	0.55					1.25
	Hardness (mg/L)	260					240
Moira Lk. - ML-5 03990556	Temp. (°C)	23.5	24.0	24.0	23.5	23.0	22.0
	D.O. (mg/L)	8.4	8.3	8.1	6.9	8.1	7.1
	pH	8.3					8.1
	Conductivity	513					512
	Ammonia (mg/L)	0.7					1.3
	Hardness (mg/L)	270					280

Date:______________________

Approved by:______________________
Jim Reid, Laboratory Supervisor

Test Conditions

Test Organism	:	Chironomus riparius	Sediment Volume (mL)	:	100
Source	:	University of Waterloo	Control/Dilution Water	:	undiluted well watera
Life Stage at Start	:	1st instar (<48h post hatch)	Overlying Water Volume (mL)	:	175
Test Type	:	Static	Test Aeration Rate	:	2-3 bubbles/second
No. Discrete Samples/Site	:	1	Photoperiod (h) light/dark	:	16/8
Number of Replicates	:	5	Temperature	:	23 ± 1 °C
Number of Organisms per Replicate	:	10	Feeding Rate	:	6 mg dry solids in 1.5 mL suspension / replicate
Number of Organisms per Treatment	:	50	Feeding Frequency	:	Daily
Sediment Depth	:	approx 3.5 cm	Test Duration	:	10 days

^a Control/Dilution Water: No chemicals added.

Test Protocol:      Biological Test Method: Test for Survival and Growth in Sediment Using the Larvae of Freshwater Midges (Chironomus tentans or Chironomus riparius). Environment Canada, Conservation and Protection. Ottawa, Ontario. Report EPS 1/RM/32.

Test Organisms:      No organisms exhibiting unusual appearance, behaviour, or undergoing unusual treatment were used in the test.

Control Sediment :      Obtained from Long Point, Ontario.

Test Conditions:      There were no unusual conditions or deviations from the test protocol.

Reference Toxicant Date

References

^a West, Inc. and D. Gulley. 1996. Toxstat Release 3.5. Western Ecosystems Technology. Cheyenne, WY,U.S.A.

Sample Identification

 

Results

Hyalella aztecaSurvival

Hyalella azteca Growth

Station	Replicate	% Surviving Organisms	Overall Average Survival(%)	SD	CV	Mean Dry wt/Organism (mg)	Overall Average Dry wt/Organism (mg)	S.D.	CV
control	A	100	98	4.472	4.563	0.383	0.318	0.050	15.814
	B	100				0.305
	C	90				0.280
	D	100				0.266
	E	100				0358
MR-Bend Bay 03990568	A	100	100	0.000	0.000	0.317	0.274	0.038	14.0394
	B	100				0.241
	C	100				0.274
	D	100				0.230
	E	100				0.306
MR-7 03990553	A	100	%	5.477	5.705	0.329	0.310	0.030	9.825
	B	100				0.280
	C	90				0.290
	D	100				0.297
	E	90				0.353
MR-8 03990542	A	100	%	5.477	5.705	0.287	0.264	0.041	15.397
	B	90				0.196
	C	100				0.292
	D	90				0.258
	E	100				0.288
MR-9 03990543	A	80	68	16.432	24.164	0.263	0.206	0.047	22.628
	B	80				0.145
	C	40				0.208
	D	70				0.179
	E	70				0.239
MR-10 03990552	A	80	84	16.733	19.920	0.333	0.277	0.047	16.777
	B	100				0.298
	C	80				0.227
	D	100				0.299
	E	60				0.230
SR-RI 03990567	A	80	90	7.071	7.857	0.375	0.330	0.048	14.476
	B	100				0.338
	C	90				0.324
	D	90				0.361
	E	90				0.252
SR-R2 03990566	A	90	92	4.472	4.861	0.308	0.321	0.044	13.627
	B	90				0.380
	C	90				0.344
	D	100				0.263
	E	90				0.311
SR-R3	A	80	90	7.071	7.857	0.303	0.299	0.054	18.032
	B	90				0.249
	C	90				0.351
	D	90				0.240
	E	100				0.353

Technician: _______________________________

Station	Replicate	% Surviving Organism	Overall Average Survival (%)	SD	CV	Mean Dry wt/Organism (mg)	Overall Average Dry wt/Organism (mg)	S.D.
Control	A	100	98	4.4772	4.563	0.383	0.318	0.050
	B	100				0.305
	C	90				0.280
	D	100				0.266
	E	100				0.358
MR-Bend Bay 03990568	A	100	100	0.000	0.000	0.317	0.274	0.038
	B	100				0.241
	C	100				0.274
	D	100				0.230
	E	100				0.306
MR-7 03990553	A	100	96	5.477	5.705	0.329	0.310	0.030
	B	100				0.280
	C	90				0.290
	D	100				0.297
	E	90				0.353
MR-8 03990542	A	100	96	5.477	5.705	0.287	0.264	0.041
	B	90				0.196
	C	100				0.292
	D	90				0.258
	E	100				0.288
MR-9 03990543	A	80	68	16.432	24.164	0.263	0.206	0.047
	B	80				0.145
	C	40				0.208
	D	70				0.179
	E	70				0.239
MR-10 03990552	A	80	84	16.733	19.920	0.333	0.277	0.047
	B	100				0.298
	C	80				0.227
	D	100				0.299
	E	60				0.230
SR-R1 03990567	A	80	90	7.071	7.857	0.375	0.330	0.048
	B	100				0.338
	C	90				0.324
	D	90				0.361
	E	90				0.252
SR-R2 03990566	A	90	92	4.472	4.861	0.308	0.321	0.044
	B	90				0.380
	C	90				0.344
	D	100				0.263
	E	90				0.311
SR-R3 03990550	A	80	90	7.071	7.857	0.303	0.299	0.054
	B	90				0.249
	C	90				0.351
	D	90				0.240
	E	100				0.353

Technician:____________________

Toxicity Test Chemistry Parameters

Sediment		0	2	5	Day 7	9	12	14
Control	Temp.(°C)	22.5	23.0	23.0	23.0	23.5	23.0	22.5
	D.O. (mg/L)	8.3	8.7	8.0	8.5	8.2	8.1	7.6
	pH	8.3						8.1
	Conductivity	497						523
	Ammonia (mg/L)	3.85						0.85
	Hardness (mg/L)	240						230
Moria R. – MR-Bend Bay 03990568	Temp. (°C)	22.5	23.0	23.0	23.0	23.5	23.0	22.5
	D.O. (mg/L)	8.2	8.7	8.3	8.4	8.2	8.4	8.5
	pH	8.4						8.2
	Conductivity	488						534
	Ammonia (mg/L)	1.85						0.3
	Hardness (mg/L)	240						280
Moria R. – MR-7 03990553	Temp. (°C)	22.5	23.0	23.0	23.0	23.5	23.0	22.5
	D.O. (mg/L)	8.5	8.8	8.3	8.2	8.3	8.2	7.8
	pH	8.3						8.0
	Conductivity	442						404
	Ammonia (mg/L)	0.35						0.4
	Hardness (mg/L)	230						210
Moria R. – MR-8 03990542	Temp. (°C)	22.5	23.0	23.0	23.0	23.5	23.0	22.5
	D.O. (mg/L)	8.2	8.4	8.3	7.6	7.7	8.0	8.0
	pH	8.3						8.4
	Conductivity	501						558
	Ammonia (mg/L)	1.55						0.3
	Hardness (mg/L)	260						280
Moria R. – MR-9 03990543	Temp. (°C)	22.5	23.0	23.0	23.0	23.5	23.0	22.5
	D.O. (mg/L)	7.7	8.7	8.0	7.9	7.7	7.5	7.8
	pH	8.3						8.1
	Conductivity	662						440
	Ammonia (mg/L)	4.75						0.45
	Hardness (mg/L)	340						200
Moria R. – MR-10 03990552	Temp. (°C)	22.5	23.0	23.0	23.0	23.5	23.0	22.5
	D.O. (mg/L)	8.3	8.5	8.1	8.5	8.1	7.9	8.4
	pH	8.3						8.4
	Conductivity	518						584
	Ammonia (mg/L)	1.85						0.55
	Hardness (mg/L	270						290
Salmon R. – SR-R1 03990567	Temp. (°C)	22.5	23.0	23.0	23.0	23.5	23.0	22.5
	D.O. (mg/L)	8.3	9.0	8.6	8.4	8.0	8.3	8.3
	pH	8.2						8.4
	Conductivity	490						627
	Ammonia (mg/L)	0.55						0.55
	Hardness (mg/L	260						340
Salmon R. – SR-R2 03990566	Temp. (°C)	22.5	23.0	23.0	23.0	23.5	23.0	22.5
	D.O. (mg/L)	8.2	8.6	7.7	8.3	8.5	8.3	8.3
	pH	8.1						8.5
	Conductivity	548						626
	Ammonia (mg/L)	1.1						0.45
	Hardness (mg/L)	240						340
Salmon R. – SR-R3 03990550	Temp. (°C)	22.5	23.0	23.0	23.0	23.5	23.0	22.5
	D.O. (mg/L)	8.3	8.8	8.3	8.4	8.2	7.7	8.5
	pH	8.4						8.5
	Conductivity	426						516
	Ammonia (mg/L)	1.5						0.3
	Hardness (mg/L	250						270

Date:____________________

Approved by:___________________
Jim Reid, Laboratory Supervisor

Test Conditions

Test Organism	:	Hyalella azteca	Sediment Volume (mL)	:	100
Source	:	AIB, Burlington, ON	Control/Dilution Water	:	undiluted well watera
Life Stage at Start	:	2-9 day old	Overlying Water Volume (mL)	:	175
Test Type	:	Static	Test Aeration Rate	:	2-3 bubbles/second
No. Discrete Samples/Treatment	:	1	Photoperiod (h) light/dark	:	16/8
Number of Replicates	:	5	Temperature	:	23 ± 1 °C
Number of Organisms per Replicate	:	10	Feeding Rate	:	2.7 mg dry solids in 1.5 mL suspension / replicate
Number of Organisms per Treatment	:	50	Feeding Frequency	:	Daily
Sediment Depth	:	3.5 cm	Test Duration	:	14 days

^a Control/Dilution Water: No chemicals added.

Test Protocol:    Biological Test Method: Test for Survival and Growth in Sediment Using the Freshwater Amphipod Hyalella azteca. Environment Canada, Conservation Protection. Ottawa, Ontario, Report EPS I/RM/33.

Test Organisms:     No organisms exhibiting unusual appearance, behaviour, or undergoing unusual treatment were used in the test. Previous 48 hour culture mortality <1%.

Control Sediment:     Obtained from Long Point, Ontario.

Test Conditions:     There were no unusual conditions or deviations from the test protocol.

Reference Toxicant Data

References

^a West, Inc, and D. Gulley. 1996. Toxstat Release 3.5. Western Ecosystems Technology, Cheyenne, WY, U.S.A.

Sample Identification

Substance	Date Collected	ESG Sample Number	Description
Control			-fine black sediment, no odour.
Young's Ck. YC-1	04/28/99	03990541	-very fine, black sediment, some plant material, no odour.
Moira R. MR-R2	04/30/99	03990564	-fine dark olive-brown sediment, large amounts of plant material (leaves, twigs), slight odour.
Moira R. MR-R3	05/12/99	03990565	-very fine, dark olive-brown sediment, slight odour. NOTE: seived through 500 µm to exclude macroorganisms and plant material
Moira R. MR-1	04/28/99	03990554	-fine, brown-black sediment, large amount of plant material, slight odour.
Moira R. MR-4	04/28/99	03990555	-fine, dark brown sediment, some plant material, slight odour.
Black R. BR-1	05/03/99	03990545	-fine to coarse (small pebbles), dark olive-brown sediment, some plant material, slight odour.
Skootamatta R SR-1	05/03/99	03990561	-medium fine, dark olive-brown sediment, approximately 50% plant material (leaves, twigs), strong odour.

^aSample MR-R3 submitted May 14/99.

Results

Hyalella azteca survival

Hyalella azteca Growth

Station	Replicate	%Surviving Organisms	Overall Average % Survival	SD	CV	Mean Dry wt/Organism (mg)	Overall Average Dry wt/Organism (mg)	S.D.	CV
Control	A	90	94	5.477	5.827	0.180	0.181	0.013	6.975
	B	90				0.176
	C	90				0.173.
	D	100				0.203
	E	100				0.173
YC-1 03990541	A	0	0	0.000	0.000	0.000	0.000	0.000	0.000
	B	0				0.000
	C	0				0.000
	D	0				0.000
	E	0				0.000
MR-R2 03990564	A	100	100	0.000	0.000	0.195	0.194	0.011	5.645
	B	100				0.181
	C	100				0.211
	D	100				0.190
	E	100				0.192
MR-R3 03990565	A	90	84	18.165902	21.62607	0.211	0.202	0.042	20.613
	B	70				0.187
	C	100				0.208
	D	60				0.145
	E	100				0.260
MR-1 03990554	A	100	82	19.235	23.458	0.207	0.216	0.032	14.823
	B	50				0.248
	C	80				0.165
	D	90				0.231
	E	90				0.229
MR-4 03990555	A	0	10	14.142	141.421	0.000	0.082	0.054	66.381
	B	0				0.000
	C	20				0.120
	D	0				0.000
	E	30				0.043
BR-1 03990545	A	80	46	40.373	87.768	0.179	0.211	0.038	18.171
	B	50				0.178
	C	0				0.000
	D	90				0.239
	E	10				0.250
SR-1 03990545	A	60	62	14.832397	23.92322	0.122	0.099	0.019	19.116
	B	80				0.115
	C	60				0.075
	D	70				0.090
	E	40				0.095

Technician : ___________________

Toxicity Test Chemistry Parameters

Sediment		0	3	5	Day 7	10	12	14
Control Long Point, ON.	Temp. (°C)	22.0	22.0	23.0	23.0	23.0	23.0	23.5
	D.O. (mg/L)	8.7	4.8	7.9	8.9	8.4	8.3	8.1
	pH	8.4						8.2
	Conductivity	490						508a
	Ammonia (mg/L)	3.95
	Hardness (mg/L)	250						240
Young's Ck.-YC-1 03990541	Temp. (°C)	22.0	22.0	23.0	23.0	23.0	23.0	23.5
	D.O. (mg/L)	8.3	8.3	8.2	8.0	8.4	8.1	7.6
	pH	8.0						6.9
	Conductivity	418						227
	Ammonia (mg/L)	3.0						0.53
	Hardness (mg/L)	150						120
Moira R.-MR-R2 03990564	Temp. (°C)	22.0	22.0	23.0	23.0	23.0	23.0	23.5
	D.O. (mg/L)	8.3	8.1	7.7	8.8	8.2	7.8	7.9
	pH	8.4						8.4
	Conductivity	603						580
	Ammonia (mg/L)	1.7						0.9
	Hardness (mg/L)	290						300
Moira R..-MR-R3 03990565	Temp. (°C)	22.0	22.0	23.0	23.0	23.0	23.0	23.5
	D.O. (mg/L)	8.5	8.1	7.7	8.7	8.3	8.1	8.1
	pH	8.5						8.6
	Conductivity	539						617
	Ammonia (mg/L)	3.85						0.45
	Hardness (mg/L)	380						360
Moira R.-MR-1 03990554	Temp. (°C)	22.0	22.0	23.0	23.0	23.0	23.0	23.5
	D.O. (mg/L)	7.8	8.1	6.0	8.9	8.2	7.9	7.8
	pH	8.2						8.5
	Conductivity	643						558
	Ammonia (mg/L)	2.3						0.25
	Hardness (mg/L)	330						330
Moira R. - MR-4 03990555	Temp. (°C)	22.0	22.0	23.0	23.0	23.0	23.0	23.5
	D.O. (mg/L)	7.2	8.9	8.2	8.8	8.3	8.3	8.2
	pH	8.3						8.6
	Conductivity	835						601
	Ammonia (mg/L)	5.45						0.43
	Hardness (mg/L)	380						350
Black R. - BR-1 03990545	Temp. (°C)	22.0	22.0	23.0	23.0	23.0	23.0	23.5
	D.O. (mg/L)	8.3	8.4	8.0	8.8	8.0	7.8	7.8
	pH	8.3						8.5
	Conductivity	559						564
	Ammonia (mg/L)	2.55						0.23
	Hardness (mg/L)	260						300
Skootamatta R.-SR-1 03990561	Temp. (°C)	22.0	22.0	23.0	23.0	23.0	23.0	23.5
	D.O. (mg/L)	7.5	8.6	7.7	8.9	8.4	7.9	8.1
	pH	8.2						8.2
	Conductivity	574						354
	Ammonia (mg/L)	4.85						0.48
	Hardness (mg/L)	300						260

^a– not measured

Date:____________________

Approved By:______________________
Jim Reid, Laboratory Supervisor

Test Conditions

Test Organism	:	Hyalella azteca	Sediment Volume (mL)	:	100
Source	:	AIB, Burlington, ON	Control/Dilution Water	:	undiluted well watera
Life Stage at Start	:	2-9 day old	Overlying Water Volume (mL)	:	175
Test Type	:	Static	Test Aeration Rate	:	2-3 bubbles/second
No. Discrete Samples/Treatment	:	1	Photoperiod (h) light/dark	:	16/8
Number of Replicates	:	5	Temperature	:	23 ± 1 °C
Number of Organisms per Replicate	:	10	Feeding Rate	:	2.7 mg dry solids in 1.5 mL suspension / replicate
Number of Organisms per Treatment	:	50	Feeding Frequency	:	Daily
Sediment Depth	:	3.5 cm	Test Duration	:	14 days

^a Control/Dilution Water: No chemicals added.

Test Protocol:     Biological Test Method: Test for Survival and Growth in Sediment Using the Freshwater Amphipod Hyalella azteca. Environment Canada, Conservation and Protection . Ottawa, Ontario. Report EPS I/RM/33.

Test Organisms:     No organisms exhibiting unusual appearance, behaviour, or undergoing unusual treatment were used in the test. Previous 48 h culture mortality <1%.

Control Sediment:     Obtained from Long Point, Ontario.

Test Conditions:     Sediment MR-R3 contained abundant small amphipods which could not be eliminated by hand picking. Sediment sample was sieved using a 500µm stainless steel mesh prior to testing. There were no other unusual conditions or deviations from the test protocol.

Reference Toxicant Data

References

^aWest, Inc. and D. Gulley. 1996. Toxstat Release 3.5. Western Ecosystems Technology. Cheyenne, WY, U.S.A.

    Sample Identification

 

Substance	Data Collected	ESG Sample Number	Description
Control			-fine black sediment, no strong odour.
Moira R. MR-Bend Bay	04/27/99	03990568	-very fine, dark brown sediment, slight odour.
Moira R. MR-7	04/29/99	03990553	-fine,dark olive-brown sediment, some plant material, slight odour.
Moira R. MR-8 (Chisholm's Mills)	04/29/99	03990542	-fine, dark brown sediment (some lumps), some shells and plant material, slight odour.
Moira R. MR-9 (Latta)	04/29/99	03990543	-fine-coarse (sandy), dark brown sediment, large amount of plant material, some molluse shells, strong odour.
Moira R. MR-10 (HWY 37)	04/29/99	03990552	-fine, dark brown-black sediment, little plant material, slight odour.
Salmon R. SR-R1	05/03/99	03990567	-very fine, olive-brown sediment, some plant material, slight odour.
Salmon R. SR-R2	05/03/99	03990566	-fine, dark brown sediment, large mounts of plant material, slight odour.
Salmon R. SR-R3	05/03/99	03990550	-fine, dark brown sediment (some rocks and pebbles), large amounts of plant material, faint odour.

Results

Chironomus riparius Survival

Chironomus riparius Growth

Technician:____________________________

Toxicity Test Chemistry Parameters

				Day
Sediment		0	2	5	7	9	10
Control	Temp. (°C)	23.0	22.5	23.0	23.0	22.0	22.0
	D.O. (mg/L)	7.3	8.4	7.9	8.1	8.4	8.7
	pH	8.5					8.2
	Conductivity	482					545
	Ammonia (mg/L)	2.63					0.48
	Hardness (mg/L)	265					265
Moira R. - MR-Bend Bay 03990568	Temp. (°C)	23.0	22.5	23.0	23.0	22.0	22.0
	D.O. (mg/L)	8.4	8.3	7.8	8.3	8.5	8.6
	pH	8.3					8.1
	Conductivity	533					564
	Ammonia (mg/L)	1.90					0.68
	Hardness (mg/L)	280					300
Moira R. - MR-7 03990553	Temp (°C)	23.0	22.5	23.0	23.0	22.0	22.0
	D.O. (mg/L)	8.4	8.1	8.2	7.6	8.5	8.8
	pH	8.3					8.3
	Conductivity	440					423
	Ammonia (mg/L)	0.45					0.48
	Hardness (mg/L)	250					210
Moira R. - MR-8 03990542	Temp (°C)	23.0	22.5	23.0	23.0	22.0	22.0
	D.O. (mg/L)	6.2	8.4	8.2	8.4	8.6	8.4
	pH	7.8					8.4
	Conductivity	514					520
	Ammonia (mg/L)	2.0					5.95
	Hardness (mg/L)	275					240
Moira R. - MR-9 03990543	Temp (°C)	23.0	22.5	23.0	23.0	22.0	22.0
	D.O. (mg/L)	6.2	8.5	8.0	8.2	7.2	8.6
	pH	7.8					8.4
	Conductivity	625					527
	Ammonia (mg/L)	3.25					6.88
	Hardness (mg/L)	345					235
Moira R. - MR-10 03990552	Temp (°C)	23.0	22.5	23.0	23.0	22.0	22.0
	D.O. (mg/L)	8.3	8.4	7.9	8.0	8.7	8.7
	pH	8.3					8.4
	Conductivity	529					512
	Ammonia (mg/L)	2.4					0.03
	Hardness (mg/L)	305					270
Salmon R. - SR-R1 03990567	Temp. (°C)	23.0	22.5	23.0	23.0	22.0	22.0
	D.O. (mg/L)	8.3	8.3	7.2	6.1	8.6	8.8
	pH	8.2					8.5
	Conductivity	519					485
	Ammonia (mg/L)	0.85					0.10
	Hardness (mg/L)	275					260
Salmon R. - SR-R2 03990566	Temp. (°C)	23.0	22.5	23.0	23.0	22.0	22.0
	D.O. (mg/L)	8.5	8.5	8.2	8.3	8.6	8.6
	pH	8.4					8.4
	Conductivity	540					562
	Ammonia (mg/L)	1.35					3.55
	Hardness (mg/L)	290					290
Salmon R.- SR-R3 03990550	Temp. (°C)	23.0	22.5	23.0	23.0	22.0	22.0
	D.O. (mg/L)	8.2	8.4	8.1	7.5	8.6	8.7
	pH	7.5					8.3
	Conductivity	495					475
	Ammonia (mg/L)	1.90					4.18
	Hardness (mg/L)	285					250

Date:__________________________

Approved by:______________________________
Jim Reid, Laboratory Supervisor

Test Conditions

Test Organism	:	Chironomus riparius	Sediment Volume (mL)	:	100
Source	:	University of Waterloo	Control/Dilution Water	:	undiluted well watera
Life Stage at Start	:	1st instar (<48h post hatch)	Overlying Water Volume (mL)	:	175
Test Type	:	Static	Test Aeration Rate	:	2-3 bubbles/second
No. Discrete Samples/Site	:	1	Photoperiod (h) light/dark	:	16/8
Number of Replicates	:	5	Temperature	:	23 ± 1 °C
Number of Organisms per Replicate	:	10	Feeding Rate	:	6 mg dry solids in 1.5 mL suspension / replicate
Number of Organisms per Treatment	:	50	Feeding Frequency	:	Daily
Sediment Depth	:	approx 3.5 cm	Test Duration	:	10 days

^a Control/Dilution Water: No chemicals added.

Test Protocol:     Biological Test Method: Test for Survival and Growth in Sediment Using the Larvae of Freshwater Midges (Chironomus tentans or Chironomus riparius). Environment Canada, Conservation and Protection. Ottawa, Ontario. Report EPS 1/RM/32.

Test Organisms:     No organisms exhibiting unusual appearance, behaviour, or undergoing unusual treatment were used in the test.

Control Sediment:     Obtained from Long Point, Ontario.

Test Conditions:     Holding time exceeded 6 weeks.

Reference Toxicant Data

References

^aWest, Inc. and D.Gulley. 1996. Toxstat Release 3.5. Western Ecosystems Technology. Cheyenne, WY, U.S.A.

Sample Identification

 

Substance	Data Collected	ESG Sample Number	Description
Control			-fine black sediment, no strong odour.
Young's Ck. YC-1	04/28/99	03990541	-very fine, black sediment, some plant material, no strong odour.
Moira R. MR-R2	04/30/99	03990564	-fine, dark olive-brown sediment, large amounts of plant material (leaves, twigs), slight odour.
Moira R. MR-R3	04/30/99	03990565	-very fine, dark olive-brown sediment, slight odour. NOTE: seived through 500 µm to exclude macroorganisms
Moira R. MR-1	04/28/99	03990554	-fine, brown-black sediment, large amount of plant material, slight odour.
Moira R. MR-4	04/28/99	03990555	-fine, dark brown sediment, some plant material, slight odour.
Black R. BR-1	05/03/99	03990545	-fine to coarse (small pebbles), dark olive-brown sediment, some plant material, slight odour.
Skootamatta R. SR-1	05/03/99	03990561	-medium fine, dark olive-brown sediment, approximately 50% plant material (leaves, twigs), strong odour.

^asample MR-R3 submitted May 14, 1999

Results

Chironomus riparius Survival

Chironomus riparius Growth

Technician:_____________________

Toxicity Test Chemistry Parameters

				Day
Sediment		0	1	3	6	8	10
Control	Temp. (°C)	22.0	23.0	23.0	23.5	23.0	23.5
	D.O. (mg/L)	8.4	8.0	8.8	7.9	8.2	8.1
	pH	8.3					8.2
	Conductivity	481					481
	Ammonia (mg/L)	2.50					0.35
	Hardness (mg/L)	240					230
Young's Ck.-YC-1 03990541	Temp. (°C)	22.0	23.0	23.0	23.5	23.0	23.5
	D.O. (mg/L)	8.8	7.9	8.7	7.9	8.1	8.3
	pH	8.0					7.4
	Conductivity	291					215
	Ammonia (mg/L)	2.8					0.35
	Hardness (mg/L)	140					90
Moira R. - MR-R2 03990564	Temp (°C)	22.0	23.0	23.0	23.5	23.0	23.5
	D.O. (mg/L)	8.6	6.1	8.8	8.1	8.2	8.0
	pH	8.5					8.3
	Conductivity	594					525
	Ammonia (mg/L)	2.3					0.4
	Hardness (mg/L)	280					250
Moira R. - MR-R3 03990565	Temp (°C)	22.0	23.0	23.0	23.5	23.0	23.5
	D.O. (mg/L)	8.4	7.5	8.7	7.6	7.7	7.5
	pH	8.4					8.2
	Conductivity	594					481
	Ammonia (mg/L)	3.6					0.4
	Hardness (mg/L)	320					260
Moira R. - MR-1 03990554	Temp (°C)	22.0	23.0	23.0	23.5	23.0	23.5
	D.O. (mg/L)	8.6	7.6	8.9	7.9	8.0	8.1
	pH	8.5					8.5
	Conductivity	573					484
	Ammonia (mg/L)	3.7					0.3
	Hardness (mg/L)	160					260
Moira R. - MR-4 03990555	Temp (°C)	22.0	23.0	23.0	23.5	23.0	23.5
	D.O. (mg/L)	8.6	8.2	8.6	8.0	8.1	8.0
	pH	8.5					8.4
	Conductivity	661					622
	Ammonia (mg/L)	8.6					0.4
	Hardness (mg/L)	360					300
Black R. – BR-1 03990545	Temp. (°C)	22.0	23.0	23.0	23.5	23.0	23.5
	D.O. (mg/L)	8.6	7.9	8.9	7.6	7.7	7.6
	pH	8.5					8.3
	Conductivity	522					524
	Ammonia (mg/L)	7.5					5.95
	Hardness (mg/L)	160					250
Skootamatta R.-SR-1 03990561	Temp. (°C)	22.0	23.0	23.0	23.5	23.0	23.5
	D.O. (mg/L)	8.2	7.4	8.8	7.7	7.5	8.0
	pH	8.4					8.3
	Conductivity	552					406
	Ammonia (mg/L)	6.9					11.35
	Hardness (mg/L)	260					180

Date:________________________

APPROVED BY:_______________________
Jim Reid, Laboratory Supervisor

Test Conditions